Search results for "Non-histone protein"
showing 7 items of 7 documents
Histone Deacetylase Inhibitors from Marine Invertebrates
2020
Simple Summary Histone deacetylases (HDACs) are enzymes that control gene expression and are involved in the onset of serious human pathologies, including cancer; hence, their inhibitors (HDACis) have received increased attention in recent years. It is known that marine invertebrates produce significant amounts of molecules showing active pharmacological properties and an extensive spectrum of biomedical applications. This review is focused on the description of the molecular, biochemical, and, where available, physiological aspects of marine invertebrate-derived compounds that possess HDACi properties, taking into consideration their possible utilization as treatment agents against differe…
Tandem affinity purification of histones, coupled to mass spectrometry, identifies associated proteins and new sites of post-translational modificati…
2015
Histones and their post-translational modifications contribute to regulating fundamental biological processes in all eukaryotic cells. We have applied a conventional tandem affinity purification strategy to histones H3 and H4 of the yeast Saccharomyces cerevisiae. Mass spectrometry analysis of the co-purified proteins revealed multiple associated proteins, including core histones, which indicates that tagged histones may be incorporated to the nucleosome particle. Among the many other co-isolated proteins there are histone chaperones, elements of chromatin remodeling, of nucleosome assembly/disassembly, and of histone modification complexes. The histone chaperone Rtt106p, two members of chr…
Comparison of DNase, DNA-polymerase and RNA-polymerase activities present in the DNA-bindung proteins of normal human dermis, epidermis, horny layer …
1978
DNA-binding proteins (DBP) of normal human dermis, epidermis, horny layer and psoriatic scales represent a tissue-specific group of mostly nuclear nonhistone proteins. To analyse their function, the different DBP fractions were examined concerning the presence of DNase, DNA-polymerase and RNA-polymerase activities. DBP of normal epidermis and horny layer contain four different DNases. One DNase of both DBP fractions is active only at pH 5.0. Three DNases of epidermal DBP are active at a pH-range from 5.0--8.5, while the corresponding DNases of horny layer-DBP are most active at pH 7.4. Probably these DNases have changed their pH-optimum during keratinisation. DBP of psoriatic scales include…
STUDIES ON NORMAL AND DENERVATED SKELETAL MUSCLE CHROMATIN
1980
Publisher Summary This chapter presents studies on normal and denervated skeletal muscle chromatin. It has been reported that the inhibitors of RNA and protein synthesis can prevent the appearance of some biochemical modifications of muscle, generally induced by denervation. This observation suggests that the gene expression of muscle fiber is under motoneuron control. On the other hand, it is well known that in eukaryotic cells exists a close relationship between chromatin properties and the gene expression. In a study, some properties of normal and 7-days denervated muscle chromatin were investigated to establish whether the changes in protein metabolism in denervated skeletal muscle are …
A chromatin-associated protein from pea seeds preferentially binds histones H3 and H4
2002
Pisum sativum p16 is a protein present in the chromatin of ungerminated embryonic axes. The purification of p16 and the isolation of a cDNA clone of psp54, the gene encoding its precursor have been recently reported [Castillo, J., Rodrigo, M. I., Marquez, J. A., Zuniga, A and Franco, L. (2000) Eur. J. Biochem.267, 2156-2165]. In the present paper, we present data showing that p16 is a nuclear protein. First, after subcellular fractionation, p16 was clearly found in nuclei, although the protein is also present in other organelles. Immunocytochemical methods also confirm the above results. p16 seems to be firmly anchored to chromatin, as only extensive DNase I digestion of nuclei allows its r…
Conserved chromosomal clustering of genes governed by chromatin regulators in Drosophila
2008
Transcriptional analysis of chromatin regulator mutants in Drosophila melanogaster identified clusters of functionally related genes conserved in other insect species.
Putative high mobility group non-histone chromosomal proteins from pea (Pisum sativum)
1991
Abstract Three putative HMG proteins, 1P, 2P and 3P have been isolated from pea ( Pisum sativum L. cv. Lincoln) nuclei by extraction with either 5% perchloric acid or 0.35 M NaCl and purified by preparative electrophoresis. The amino acid analysis showed many of the typical features of the HMG proteins, although 1P and 2P possess a somewhat reduced content of acidic amino acids and 3P has less than 20% basic amino acids. Peptide mapping with Staphylococcus aureus V8 protease suggested that none of the proteins are proteolytic products of histone H1.